Automated Karyotyping of Metaphase Cells with Touching Chromosomes
نویسندگان
چکیده
منابع مشابه
Automated Karyotyping of Metaphase Cells with Touching Chromosomes
Since the birth of the automated karyotyping systems by the aid of computers, building a fully automated chromosome analysis system has been an ultimate goal. Along with many other challenges, automating chromosome classification and segmentation has been a major challenge especially due to overlapping and touching chromosomes. The earlier reported methods have limited success as they are sensi...
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The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes co...
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Previous studies of the structure of metaphase chromosomes have relied heavily on electron micrography and have revealed the existence of a 10-nm unit fiber that is thought to generate the native 23-30-nm fiber by higher order folding. The structural relationship of these metaphase fibers to the interphase fiber remains obscure. Recent studies on the digestion of interphase chromatin have revea...
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Metaphase chromosomal and interphase chromatin proteins from cells of two species have been compared by polyacrylamide gel electrophoresis. Consistent, common changes in the quantitative distribution of the nonhistone chromosomal proteins are observed in both species. Proteins of ca. 65,000 and 68,000 MW are enriched in interphase chromatin while proteins of ca. 50,000 and 200,000 are more prom...
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ژورنال
عنوان ژورنال: International Journal of Computer Applications
سال: 2011
ISSN: 0975-8887
DOI: 10.5120/3700-5175